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Server 2012 R2 File Server Stops Responding to SMB Connections

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Hi There,

Massive shot in the dark here but I am struggling with a pretty major issue atm.  We have a production file server that is hosted on the following:

Dell MD 3220i -> iSCSI -> Server 2008 R2 Hyper-v Cluster -> Passthrough Disk -> Server 2012 R2 File Server VM

Essentially 3 times now, roughly a month or so apart.  The file server stops accepting connections.  During this time, the server is perfectly accessible through rdp or with a simple ping.  I can browse the files on the server directly but no-one appears to be able to access the shares over SMB.  A reboot of the server fixes the issue.  

As per a KB article I removed nod antivirus from the server to rule out a conflicting filter mode driver after the second fault.  Sadly yesterday it happened again.

The only relevant errors in the servers log files are:

SMB Server Event ID 551

SMB Session Authentication Failure Client Name: \\192.168.105.79 Client Address: 192.168.105.79:50774 User Name: HHS\H6-08$ Session ID: 0xFFFFFFFFFFFFFFFF Status: Insufficient server resources exist to complete the request. (0xC0000205) Guidance: You should expect this error when attempting to connect to shares using incorrect credentials. This error does not always indicate a problem with authorization, but mainly authentication. It is more common with non-Windows clients. This error can occur when using incorrect usernames and passwords with NTLM, mismatched LmCompatibility settings between client and server, duplicate Kerberos service principal names, incorrect Kerberos ticket-granting service tickets, or Guest accounts without Guest access enabled

and

SMB Server event ID 1020
File system operation has taken longer than expected.

Client Name: \\192.168.105.97
Client Address: 192.168.105.97:49571
User Name: HHS\12J.Champion
Session ID: 0x2C07B40004A5
Share Name: \\*\Subjects
File Name:
Command: 5
Duration (in milliseconds): 176784
Warning Threshold (in milliseconds): 120000

Guidance:

The underlying file system has taken too long to respond to an operation. This typically indicates a problem with the storage and not SMB.

I have checked the underlying disk/iscsi/network hyper-v cluster for any other errors or issues, but as far as I can tell everything is fine. 

Is it possible that something else is left over from the NOD antivirus installation?  

Looking for suggestions on how to troubleshoot this further.

Thanks



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