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Windows 2012 R2 Fileserver - Files open sometimes slow

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We have problems - file open sometimes slow. Fileserver isWindows 2012 R2 (all updates) and Window 7 clients. The  Fileservers are virtual and running von VMWare vShpere 5.1. (paravirtual, vmxnet3 NICs) On the fileserver is no bottleneck CPU, RAM, NIC, DISC, TCP-Connections visable. Performance Reports are alway green, system resports are alway green. On some volumes dedublication ist enabled but this issueoccurson all volumes. Nothing interessting to see in the eventlogs.

The issue lookfollows: A useropen a filethat takes long time.Another useropensother files and has no problem at the same time! Especiallyfilesare affected thatotherusers have in accessat that time. This affects allFiles(from simple textfilestoExcel,Word,... )

The followingthingshave beentriedwithoutsuccess:
- install the system again with default smb settings (without AV, ... )
- disable SMBv2 on Client side
- stop backup software
- move the disc files of the VM (Fileserver) to an other storage system
- move the VM to an other host
- and settings from the performance tuning guide


Does anyone have suggestions
how to geta solution?


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